Harness the power of flow cytometry for EV analysis

Finally, you can take full advantage of the power of flow-based methods for analyzing extracellular vesicles (EVs) with SBI’s exclusive ExoFlow-ONE Gemstone dyes. By specifically labeling internal EV components with one of these proprietary, high quantum efficiency dyes, you can achieve near single-vesicle resolution* for more powerful flow cytometry and FACS studies, and uncover greater insights into EV biology.

ExoFlow-ONE EV Labeling Kits:

• Directly detect EVs by specifically labeling internal EV components
• Achieve near single-vesicle visualization* with our proprietary ExoFlow-ONE dyes that deliver
  • High quantum efficiency
  • Negligible background signal when not bound to EVs
• Accurately estimate the size of labeled EVs and ensure proper run-to-run calibration with the included size standards
• Learn more from a single sample by multiplexing with our range of spectrally separated ExoFlow-ONE dyesThe ExoFlow-ONE Garnet

The ExoFlow-ONE Garnet Far Red Gemstone Dye Kit comes with enough labeling reagents for 25 reactions**. Each kit also includes 1.5 mL of silica bead size standards (110 nm – 1300 nm), two of which are fluorescent. The silica beads, sourced from Apogee Flow Systems, are specifically designed to produce a refractive index and light scattering patterns similar to biological particles, enabling more accurate estimation of EV particle size.

*Requires a specialized flow cytometry imager capable of high-resolution vesicle analysis (e.g. Amnis® ImageStreamX Mark II, Apogee Micro-PLUS).
**Each reaction is defined as 1 µL of working dye combined with 200 – 500 µg of EV protein resuspended in 500 µL of buffer.

Choose the right ExoFlow-ONE dye for your project